Bioremediation of Solid and Liquid Wastes: Biosorption of Heavy Metal by Acclimated Microorganisms

In this study, the samples were collected from selected 40 sites of Uttarakhand (84 samples). The physico-chemical and microbial analysis was done according to standard method. The pH, temperature, colour, Turbidity, DO, BOD, COD, TH, TDS, chloride, fluoride, sulphate, phosphate, nitrate and heavy metal concentration was measured by standard methods. The results of physico-chemical analysis showed that waste water had more concentration than acceptable limit fixed by ICMR, WHO, EPA and APHA and river water had some parameters in limits and another have beyond the limit. The heavy metals viz. Zn, Mn, Cu, Co, Cr, As, Ni, Pb, Cd were determined in both solid and liquid wastes. The eight samples were selected, having maximum heavy metal concentrations, for the bioremediation study. The microbes were isolated from selected samples and characterized by microscopic,morphological and biochemical methods. Seven microbes were selected and they were allowed to grow in heavy metal ammended media for acclimatization, most potent were used for biosorption study. The gram +ve bacteria were found potential Bioadsorbant.