Environmental Monitoring of Bacteria - Methods in Biotechnology Softcover reprint of hardcover 1st ed. 1999 edition

Jacket Description/Back: In Environmental Monitoring of Bacteria, Clive Edwards and his distinguished panel of researchers bring together a collection of detailed molecular methods for the environmental monitoring of bacterial populations. These state-of-the-art methods include techniques for the recovery of cells, for the analysis of whole cells, and for the molecular study of target species and heterogeneous populations. Many of the methods enable bacteria to be monitored in their natural environments without culturing, and specifically address the difficult problem that only a small proportion of bacteria in any ecosystem can be cultured by traditional methods. In addition to providing many eminently reproducible methods, the distinguished contributors review the problems of monitoring bacteria in natural environments and discuss the principles by which the methods work. Taking full advantage of the exciting research in recent years that has challenged some long-held principles of traditional microbiology, Environmental Monitoring of Bacteria gives timely and practical access to powerfultechniques that allow the study of bacterial ecology as communities, as single cells, or at the molecular level. Table of Contents: Some Problems Posed by Natural Environments for Monitoring Microorganisms, Clive Edwards. Sampling Sediment and Soil: Use of Coring Devices, Roger Pickup, Glenn Rhodes, and Grahame Hall. Sampling Water Bodies: Tangetial Flow Filtration, Roger Pickup, Helen Mallinson, and Glenn Rhodes. Dielectrophoresis, D. W. Pimbley, P. D. Patel, and C. J. Robertson. Flow Cytometry and Cell Sorting: Rapid Analysis and Separation of Individual Bacterial Cells from Natural Environments, Jonathan Porter. Magnetic Particle-Based Separation Techniques for Monitoring Bacteria from Natural Environments, Jonathan Porter and Roger Pickup. DNA Extraction from Natural Environments, Kenneth D. Bruce, Peter Strike, and Donald A. Ritchie. Automated Sequencing of DNA Retrieved from Environmental Samples, Mathew Upton. Analysis of DNA Sequences, Mathew Upton. Fluorescent Polymerase Chain Reaction/Restriction Fragment Length Polymorphism Monitoring of Genes Amplified Directly from Bacterial Communities in Soils and Sediments, Kenneth D. Bruce and Mark R. Hughes. Recovery and Analysis of Ribosomal RNA Sequences from the Environment, Ian M. Head. Application of Denaturing Gradient Gel Electrophoresis to Microbial Ecology, Richard Hastings. Reporter Gene Expression for Monitoring Microorganisms in the Environment, James R. Firth. Characterizing Microorganisms in the Environment by Fatty Acid Analysis, Ian P. Thompson, Mark J. Bailey, and Andrew K. Lilley. Fluorescent In Situ Hybridization and the Analysis of the Single Cell, Anthony G. O'Donnell and Andrew S. Whiteley. Specific Detection, Viability Assessment, and Macromolecular Staining of Bacteria for Flow Cytometry, Jonathan Porter. Confocal Laser Scanning Microscopy of Environmental Samples, David Lloyd, Anthony J. Hayes, and James R. Ralphs. Monitoring Microbial Activities Using Membrane Inlet Mass Spectrometry, James R. Firth and Clive Edwards. Experimental Biofilms and Their Applications in the Study of Environmental Processes, Joanna C. Raynerand Hilary M. Lappin-Scott. Establishment of Experimental Biofilms Using the Modified Robbins Device and Flow Cells, Luanne Hall-Stoodley, Joanna C. Rayner, Paul Stoodley, and Hilary M. Lappin-Scott. Index. Review Quotes: "This book looks at state-of-the-art techniques for monitoring bacteria in natural environments, offers step-by-step instructions to ensure readily reproducible results, and provides numerous tips on methods for optimizing results."-Environmental Science & Technology, July 1, 1999 "All contributions conformed to a given structure and so the individual sections have turned out to be compact as well as comprehensive and clear. They describe the principles of different techniques as well as their applications and limitations and most of them contain easy-to-follow protocols providing immediate access to a given method." - International Journal of Hygiene and Environmental HealthPublisher Marketing: Env1r0nmenta1 ha5 m1cr0610109y emer9ed an extreme1y act1ve, - and 1mp0rtant area 0f re5earch dur1n9 the few year5 and ha5 cha1- 1en9ed 50me 10n9-he1d 0f F0r examp1e, the num6er5 0f c010ny-f0rm1n9 un1t5 06ta1ned 0n a9ar med1a have way5 6een a55umed t0 ref1ect the num6er5 0f 6acter1a pre5ent any 91ven 5amp1e. H0wever, n0w kn0wn that many 6acter1a the 1a60rat0ry ad0pt unu5ua1 d0rmant 5tate5 when 5u6jected t0 the nutr1ent-1- c0nd1t10n5 1ted c0mm0n many ec05y5tem5, wh1ch, turn, make5 the5e 6- ter1a t0 cu1ture. 7h15 0ur current t0 cu1ture the va5t maj0r1ty 0f6acter1a kn0wn t0 re51de natura1 env1r0nment5. 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